Photobioreactors
Precise Phototrophic Cultivation of Algae and Cyanobacteria
About...

Photobioreactors FMT 150/400, FMT 150/1000 and
FMT 150/3000 feature a unique combination of the
cultivator and computer-controlled monitoring device.
FMT 150/400, FMT 150/1000 and FMT 150/3000 differ
in the capacity of their cultivation vessels - 400 ml, 1000 ml and 3000ml respectively. All Photobioreactor models may be supplied with a number of useful accessories - sensors, stirrers, pumps, etc.

Key Features

  • Integrated Double-Modulation Fluorometer with online measurement and recording of chlorophyll fluorescence parameters, such as F0, FT, FM, F’M, (F’M-FT)/F’M
  • Integrated online optical density measurement (OD680, OD720)
  • Continuous temperature control
  • Continuous pH monitoring


  • O2 electrode to measure concentration of dissolved O2
  • (optional)
  • CO2 electrode to determine dissolved CO2 concentration
  • (optional)
  • Highly precise gas mixing system for aeration (optional)
  • Monitoring of medium consumption during a turbidostatic cultivation (optional)

Photobioreactors


Photobioreactor FMT 150 features a unique combination of the cultivator and computer controlled monitoring device. The Photobioreactor FMT 150 combines a flat cultivation vessel with the built-in fluorometer and densitometer. It is primarily designed for high-content, precise phototrophic cultivation of algae, cyanobacteria and bacteria.

Light intensity and spectral characteristic as well as temperature and aeration gas composition can be set with high accuracy. Cultivation conditions can be dynamically varied according to user-defined protocol and continuously online monitored. Programmable light, temperature, gas, and medium regime can oscillate with various amplitudes and frequencies according to user-defined time steps from milliseconds to hours.

Continuous flow turbidostatic cultivation can be used for the stabilization of the suspensions and optical density control. In addition to the turbidostatic pump, up to 7 peristaltic pumps for different chemostat or pH-stat cultivation can be linked to the Photobioreactor for highly precise control of cultivation conditions.

The growth of the cultures is monitored by the integrated densitometer (OD680, OD720). Chlorophyll content of the culture can also be monitored continuously by the difference of optical densities at 680 and 720 nm. The instantaneous physiological state of the culture is measured by the Photosystem II quantum yield FV/FM.

Photobioreactor function can be enhanced by the additional gas modules: Gas Mixing System GMS 150 providing optimal control of input gas concentration; and gas analyzer MS GAS-100 intended for complex analyses of gases and volatiles including isotopes, solvents and volatile organics in headspace and liquid phase as well.

Cultivation


  • Autoclavable flat glass vessel - 3 different working volumes available: 400 ml, 1000 ml, 3000 ml
  • Programmable light, temperature, gas, and medium regime. Time steps from milliseconds to hours.
  • Both static and changeable temperature
  • Bubble humidifer for stable culture volume
  • Controlled flow rate and composition of the sparging gas with GMS 150
  • Magnetic stirring
  • Anaerobic cultivation via gas tight lid and N2 sparging
  • Turbidostat, chemostat or pH-stat cultivation control

LED Illumination


  • Bi-color LED panel (white-red or blue-red typically) with separately controllable channels. Other color combination on request
  • Homogeneous illumination over the whole flat vessel
  • Controllable light intensity up to 1,500 µmol.m-2.s-1 (optionally up to 3,000 µmol.m-2.s-1)
  • Both static and fluctuating light regimes. Day/night cycles
  • Additional front light panel for higher intensity or different light color

Application


  • Precise cultivation of photoautotrophic microorganisms.
  • Cultivation conditions optimization and yield improvement.
  • Photosynthesis research.
  • Stress physiology, detection of biotic and abiotic stress.
  • Study of diurnal or metabolic rhythms.
  • Study of secondary metabolites production.
  • Eco-toxicological research.
  • Ecological research, microorganisms interactions, population dynamic.
  • Environmental modeling.


Photobioreactor Versions


Volume Version Standard Intensity/ Light Upgrade Color Temperature Range
400 ml PBR FMT 150/400-RW 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, WHITE 5-75°C
PBR FMT 150/400-RB 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, BLUE 5-75°C
PBR FMT 150/400-CUST 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 on request 5-75°C
1000 ml PBR FMT 150/1000-RW 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, WHITE 10-75°C
PBR FMT 150/1000-RB 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, BLUE 10-75°C
PBR FMT 150/1000-CUST 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 on request 10-75°C
3000 ml PBR FMT 150/3000-RW 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, WHITE 10-75°C
PBR FMT 150/3000-RB 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, BLUE 10-75°C
PBR FMT 150/3000-CUST 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 on request 10-75°C

IMPORTANT NOTE: Additional accessories requested for full functionality.

Specifications

  • Online Measured Parameters: Chlorophyll fluorescence, OD, pH, temperature, O2, CO2, medium consumption during turbidostatic cultivation
  • Vessel Capacity:
  • 400 ml (FMT 150/400)
    1,000 ml (FMT 150/1000)
    3,000 ml (FMT 150/3000)

  • Precision Controlled Temperature (depending on ambient conditions):
  • 5–75 °C (FMT 150/400)
    10–75 °C (FMT 150/1000 and FMT 150/3000)

  • Cooling and Heating System:
  • 200 W Peltier element (FMT 150/400 and FMT 150/1000)
    400 W Peltier element (FMT 150/3000)

  • LED Lighting: Light intensity adjustable in the full range up to 1,500 µmol.m-2.s-1 (optionally up to 3,000 µmol.m-2.s-1).
    Bi-color light panels available (separate color control)
  • Light Path:
  • 24 mm (FMT 150/400)
    61 mm (FMT 150/1000 and FMT 150/3000)

  • Light Regime: Static or dynamic
  • Optical Density Measurement: Real time measurement of OD by two far-red LEDs (680 nm, 720 nm). Time intervals of OD measurements may be specifed
  • Chlorophyll Fluorescence Measurement: Blue excitation light and red-orange excitation light
  • Optical Path for OD and Chlorophyll Fluorescence Measurement: 24 mm
  • Controlled Flow Rate and Concentration of Sparging Gas: Optional
  • Pumps:Up to 8 peristaltic pumps
  • Optional Probes:ph/temperature, O2, CO2
  • BIOS:Upgradeable firmware
  • Communication Port: USB A-B
  • Material: Glass, stainless steel, silicon gasket
  • Dimension:
  • 41.5 x 35 x 31 cm (FMT 150/400 and FMT 150/1000)
    50 x 35 x 31 cm (FMT 150/3000)

  • Weight:
  • 15.5 kg (FMT 150/400)
    17.5 kg (FMT 150/1000)
    28 kg (FMT 150/3000)

  • Electrical: 90-240 VAC

Accessories

Photobioreactor Control Unit with BASIC Software *

Includes control unit (PC notebook ) + BASIC version software for Photobioreactor control, user-defined protocol writing, data collection and visualization in tables or graphs.

Photobioreactor Control Unit with ADVANCED Software *

Includes control unit (PC notebook ) + ADVANCED version software for Photobioreactor control, user-defined protocol writing, data collection and visualization in tables or graphs.

Additional Software License (for Both BASIC and ADVANCED Versions)

A separate license required for Photobioreactor software installation to each additional PC/laptop.

Light Upgrade

To increase intensity of actinic light in the Photobioreactor. Maximum actinic light up to 3,000 µmol(photons)/m2.s (1,500 µmol(photons)/m2.s for each color).

Magnetic Stirrer V-200

Magnetic stirrer with 35 mm long (Ø 6 mm) Teflon-coated magnetic bar that is positioned in a vertical plane of the front glass panel inside the Photobioreactor. The speed of rotation is adjustable from 60 to 600 rpm (10 – 100 %) by the front panel of the control unit or by the PBR software. Its use is essential when the agitation by air or gas bubbles cannot be used, e.g. when the photosynthetic oxygen evolution or respiratory oxygen uptake are measured or when growing the culture anaerobically without gas supply. It can be also used to reduce cell sedimentation and clumping in addition to agitation by gas bubbling. It is highly recommended with use of the O2 probe and CO2 probes.

Magnetic Stirrer V-230

Magnetic stirrer V-230 is intended for use with Photobioreactor FMT 150/3000 (3L volume cuvette). Teflon-coated magnetic bar is positioned in a vertical plane of the front glass panel inside the Photobioreactor. The speed of rotation is adjustable from 60 to 600 rpm.

High-Precision Gas Mixing System GMS 150

The standard version GMS 150 includes two channels (channel 1 for Air-N2; channel 2 for CO2). Preferably it is intended for use with PSI Multi-Cultivators, small-volume Photobioreactors, large-volume Photobioreactors and Growth Chambers. High-precision GMS 150 can optionally hold up to four channels.

NEW! - Gas Mixing System GMS 150-MICRO

GMS 150-MICRO includes two channels (channel 1 for Air-N2; channel 2 for CO2); its maximum flow is 5L/min. Preferably it is intended for use with Multi-Cultivators and small-volume Photobioreactors. Optionally the system can hold up to four channels.

Gas Analyzing System GAS 150

Enables measuring CO2 at the cultivation vessel output. If connected to CO2 electrode, it also enables measuring CO2 in the cultivated suspension.

Turbidostat Module

Includes Peristaltic Pump PP 600 and supporting control software. The module enables continuous-flow turbidostatic cultivation. In the basic setup, the Photobioreactor optical sensing unit monitors the optical density (OD 680, OD 720) of the culture. The Peristaltic Pump adds medium accordingly to keep the culture turbidity constant.

Chemostat Module

Includes two Peristaltic Pumps PP 600 and software supporting control software. One pump serves adding acid, the second pump adds base. In chemostat mode pH value of the cultivation media can be continuously adjusted as either acid or base is added to the cultivation vessel to keep pH level on defined level.

pH-Stat / Turbidostat Module

Includes Peristaltic Pump PP 600 with one gas valve and supporting control software. The Module enables either turbidostat mode cultivation or pH-stat mode cultivation (possibility of gas regulation - pH-stat management via changing CO2 flow rate). CO2 container is not included.

O2 Module

To measure concentration of dissolved O2 (includes the electronic module, sensor and cables).

O2 Sensor

Supplementary sensor to O2 measuring module (without cable).

O2 Membrane Kit

O2 membrane kit for O2 probe. Includes: 4 membrane bodies, 1 O-ring set, 25 ml electrolyte.

CO2 Module

To determine the dissolved carbon dioxide concentration in the medium (includes the electronic module, sensor and cables).

CO2 Sensor

Supplementary sensor to CO2 measuring module (without cable).

CO2 Membrane Kit

CO2 membrane kit for CO2 probe. Includes: 4 membrane bodies, 1 O-ring set, 25 ml electrolyte.

pH / Temperature Module

To measure pH and temperature (includes the electronic module, sensor and cables).

pH/T Sensor

Supplementary sensor to pH/Temperature Module (without cable).

Spare Part Kit for Turbidostat / Chemostat / pH-Stat Module

Package includes 5 pieces of tubing (autoclavable A-60-F 4x6 mm) and 8 corresponding connectors (4 pcs MTLL240-J1A + 4 pcs FTLL240-1).

PWM Pump (for Gas or Liquid)

PWM pump can be used for pumping liquid or gas into the cultivation cuvette; it can be regulated withing a range of 1-100 % of total output. The pump has diverse uses: (1) Controlled air bubbling in the cultivation vessel; (2) Single gas input into the vessel; (3) When connected to the vessel inlet and outlet, it can be used for suspension mixing (bubbling) in anaerobic conditions.

Additional Cable to pH/T Sensor

Additional cable and connector for pH/T measuring sensor.

Additional Cable to O2 Sensor

Additional cable and connector for O2 measuring sensor.

Probe Cap

Stainless plug that enables closing unused probe opening in the Photobioreactor cuvette lid.

Pipetting Insert to Top Connector

Stainless pipetting insert to the top connector on Photobioreactor cuvette lid (intended for pipetting; enables also complete closing).

Air Pump with Bubble Interruption Valve

Air pump for culture bubble stirring. Supplemented with a valve, which interrupts bubbling during single measurements, and thus allows for more precise readings.

External Light Panel

To accommodate specific needs of different organisms or to increase illumination intensity. External light panel is offered in three versions: single color (E-1), single color with additional IR LEDs (E-2), and bicolor (E-3).

Spare Aeration Set for FMT 150/400 and FMT 150/1000

The set includes: 5 stainless tubes of different length (2 long tubes + U-tube used for gas sparging, medium tube used for sampling, short tube used for overflowing) + 4x plastic connectors 5/16" BSFTLL Female.

Supplementary Vessel (standard model)

Suitable for cultivation in the range of 18 - 50 °C. Made from stainless steel and glass, it includes the frame, lid and glass body. Manufactured in a 400 ml or 1000 ml version.

Supplementary Glass Part to Cultivation Vessel

Includes just the glass body (not the frame, bottom, or cover). Made of boiling glassware, thickness 3.3 mm. Delivered both for 400 ml and 1000 ml vessels.

Gasket Kit for Cultivation Vessel

Includes: 5 pcs Silicone gasket for vessel bottom part, 5 pcs Silicone gasket for vessel top part, 5 pcs Silicone O-ring 75/3, 15 pcs Silicone O-ring 16/2. Delivered both for 400 ml and 1000 ml vessels.

Enhanced Vessel

Provides wider temperature range for cultivation. It is suitable for cultivation in the range of 5-75 °C (10-75°C). Made from nickel-plated brass covered with ceramic layer. Manufactured in a 400 ml or 1000 ml or 3000 ml version.

Spare Part Kit for Photobioreactor FMT 150

Kit includes: bulk connection plastics 45pc, one-way valves 5pc, double-check valve 5pc, stainless bushing with silicone ring 2pc, rubber gasket 5pc, o-rings 5pc, silicone tubing 10 m.

Additional Protective Glasses

Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge.

Shelf Unit

Heavy duty shelf unit can accommodate and organize the Photobioreactor with all necessary accessories.

Photobioreactor FMT 150

Volume Version Standard Intensity / Light Upgrade Color Temperature Range Price
400 ml PBR FMT 150/400-RW 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, WHITE 5-75°C 11.999 €
PBR FMT 150/400-RB 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, BLUE 5-75°C 11.999 €
PBR FMT 150/400-CUST 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 on request 5-75°C Please enquire €
1000 ml PBR FMT 150/1000-RW 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, WHITE 10-75°C 14.999 €
PBR FMT 150/1000-RB 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, BLUE 10-75°C 14.999 €
PBR FMT 150/1000-CUST 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 on request 10-75°C Please enquire €
3000 ml PBR FMT 150/3000-RW 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, WHITE 10-75°C 19.999 €
PBR FMT 150/3000-RB 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 RED, BLUE 10-75°C 19.999 €
PBR FMT 150/3000-CUST 1.500 µmol.m-2.s-1/ 3000 µmol.m-2.s-1 on request 10-75°C Please enquire €

Accessories

Version Characteristics Price
Light Upgrade for FMT 150/400
and FMT 150/1000
Light intensity upgrade – actinic light up to 3,000 µmol.m-2.s-1 (1,500 µmol.m-2.s-1 for each color). 1.990 €
Light Upgrade for FMT 150/3000 Light intensity upgrade – actinic light up to 3,000 µmol.m-2.s-1 (1,500 µmol.m-2.s-1 for each color). 2.490 €
FMT 150 or MC 1000 Control Unit
with Software Version BASIC *
Includes control unit (notebook with LCD, integrated keyboard and mouse) + BASIC version software for FMT 150/MC 1000 control, user-defined protocol writing, data collection, and visualization in tables or graphs. 2.490 €
FMT 150 or MC 1000 Control Unit
with Software Version ADVANCED *
Includes control unit (notebook with LCD, integrated keyboard, and mouse) + ADVANCED version software for FMT 150/MC 1000, user-defined protocol writing, data collection, and visualization in tables or graphs. 2.990 €
Additional Software License
(for BASIC and ADVANCED version)
A separate license required for Photobioreactor software installation to each additional PC/laptop. 300 €
Turbidostat Module Includes Peristaltic Pump PP 600 and supporting control software. The module enables continuous-flow turbidostatic cultivation. 1.990 €
Chemostat Module Includes two Peristaltic Pumps PP 600 and supporting control software. One pump serves adding acid, the second pump adds base. 2.590 €
pH-Stat / Turbidostat Module Includes Peristaltic Pump PP 600 with one gas valve and supporting control software. The Module enables either turbidostat mode cultivation or pH-stat mode cultivation (possibility of gas regulation - pH-stat management via changing CO2 flow rate). CO2 container is not included. 2.390 €
Spare Part Kit for for Turbidostat / Chemostat/ pH-Stat Module * Package includes 5 pieces of tubing (autoclavable A-60-F 4x6 mm) and 8 corresponding connectors (4 pcs MTLL240-J1A + 4 pcs FTLL240-1). 45 €
PWM Pump Pump with diverse uses: it can be used for pumping liquid or gas into the cultivation cuvette; it can be regulated withing a range of 1-100 % of total output. 680 €
O2 Module
(sensor included) *
To measure concentration of dissolved O2 (includes the electronic module, sensor and cables). 2.490 €
O2 Sensor * Supplementary sensor to O2 module (without cable). 1.400 €
O2 Membrane Kit * O2 membrane kit for O2 probe. Includes: 4 membrane bodies, 1 O-ring set, 25 ml electrolyte. 310 €
CO2 Module
(sensor included) *
To determine concentration of dissolved CO2 (includes the electronic module, sensor and cables). 3.790 €
CO2 Sensor * Supplementary sensor to CO2 module (without cable). 2.490 €
CO2 Membrane Kit * CO2 membrane kit for CO2 probe. Includes: 4 membrane bodies, 1 O-ring set, 25 ml electrolyte. 360 €
pH/Temperature Module
(sensor included) *
To measure pH and temperature of the cultivated suspension (includes the electronic module, sensor and cables). 1.690 €
pH/T Sensor * Supplementary pH/T sensor for measuring pH in the cultivated suspension (without cable). 460 €
Additional Cable to pH/T Sensor * Additional cable and connector to pH/T measuring sensor. 250 €
Additional Cable to O2 Sensor * Additional cable and connector to O2 measuring sensor. 250 €
Probe Cap Stainless plug that enables closing unused probe opening in the Photobioreactor cuvette lid. 25 €
Pipetting Insert to Top Connector Stainless pipetting insert to the top connector on Photobioreactor cuvette lid (intended for pipetting; enables also complete closing). 40 €
Spare Aeration Set for
FMT 150/400 and FMT 150/1000
The set includes: 5 stainless tubes of different length (2 long tubes + U-tube used for gas sparging, medium tube used for sampling, short tube used for overflowing) + 4x plastic connectors 5/16" BSFTLL Female. 79 €
Magnetic Stirrer V-200 Magnetic stirrer with 35 mm long Teflon-coated magnetic bar positioned in a vertical plane of the front glass panel inside the Photobioreactor. The speed of rotation is adjustable from 60 to 600 RPM. 990 €
Magnetic Stirrer V-230 Magnetic stirrer V-230 is intended for use with Photobioreactor FMT 150/3000 (3L volume cuvette). Teflon-coated magnetic bar positioned in a vertical plane of the front glass panel inside the Photobioreactor. The speed of rotation is adjustable from 60 to 600 rpm. 1.250 €
Air Pump with
Bubble Interruption Valve
Air pump for culture bubble stirring. Supplemented with a valve which interrupts bubbling during single measurements. 230 €
External Light Panel E-1 External single-color light panel to be mounted to the Photobioreactor's front part. Price to be enquired €
External Light Panel E-2 External light panel - single-color with additional IR LEDs. Price to be enquired €
External Light Panel E-3 External light panel - bi-color (separate color control, no pulse mode). Price to be enquired €
Supplementary Vessel
(standard model, 400 ml) *
Supplementary standard vessel is made from stainless steel and glass and is suitable for cultivation in the range of 18 ºC to 50 ºC. Vessel volume 400 ml. 930 €
Supplementary Vessel
(standard model, 1000 ml) *
Supplementary standard vessel is made from stainless steel and glass and is suitable for cultivation in the range of 18 ºC to 50 ºC. Vessel volume 1000 ml. 1.060 €
Supplementary Vessel
(standard model, 3000 ml) *
Supplementary standard vessel is made from stainless steel and glass and is suitable for cultivation in the range of 18 ºC to 50 ºC. Vessel volume 3000 ml. 1.690 €
Supplementary Glass Part to Cultivation Vessel (400 ml) * Includes just the glass body (not the frame, bottom, or cover). Made of boiling glassware, thickness 3.3 mm. 320 €
Supplementary Glass Part to Cultivation Vessel (1000 ml) * Includes just the glass body (not the frame, bottom, or cover). Made of boiling glassware, thickness 3.3 mm. 360 €
Supplementary Glass Part to Cultivation Vessel (3000 ml) * Includes just the glass body (not the frame, bottom, or cover). Made of boiling glassware, thickness 3.3 mm. 580 €
PBRG-400 - Gasket Kit for Cultivation Vessel (400 ml) * Includes: 5 pcs Silicone gasket for vessel bottom part, 5 pcs Silicone gasket for vessel top part, 5 pcs Silicone O-ring 75/3, 15 pcs Silicone O-ring 16/2. 99 €
PBRG-1000 - Gasket Kit for Cultivation Vessel (1000 ml) * Includes: 5 pcs Silicone gasket for vessel bottom part, 5 pcs Silicone gasket for vessel top part, 5 pcs Silicone O-ring 75/3, 15 pcs Silicone O-ring 16/2. 99 €
Enhanced Vessel (400 ml) * Made from nickel-plated brass covered with ceramic layer, the enhanced vessel is suitable for cultivation in the range of 5 ºC to 75 ºC. Vessel volume 400 ml. 960 €
Enhanced Vessel (1000 ml) * Made from nickel-plated brass covered with ceramic layer, the enhanced vessel is suitable for cultivation in the range of 10 ºC to 75 ºC. Vessel volume 1000 ml. 1.150 €
Supplementary Lid to Cultivation Vessel * 3-connector lid to the cultivation vessel. 200 €
Spare Part Kit for Photobioreactor FMT 150 * Kit includes: bulk connection plastics 45pc, one-way valves 5pc, double-check valve 5pc, stainless bushing with silicone ring 2pc, rubber gasket 5pc, o-rings 5pc, silicone tubing 10 m. 75 €
Shelf Unit Heavy duty shelf unit can accommodate and organize the Photobioreactor with all necessary accessories. 1.090 €
Gas Mixing System GMS 150 The standard version GMS 150 includes two channels (channel 1 for Air-N; channel 2 for CO2). Preferably it is intended for use with PSI Multi-Cultivators, small-volume Photobioreactors, large-volume Photobioreactors and Growth Chambers. 8.680 €
Gas Mixing System GMS 150-MICRO GMS 150-MICRO includes two channels (channel 1 for Air-N; channel 2 for CO2); its maximum flow is 5L/min. Preferably it is intended for use with Multi-Cultivators and small-volume Photobioreactors. 5.990 €
Gas Analyzing System GAS 150 Intended for measuring CO2 at the cultivation vessel output. If connected to CO2 electrode, it also enables measuring CO2 in the cultivated suspension. 11.890 €
Additional Protective Glasses * Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge. 19 €