Multi-Cultivators
Precise Phototrophic Cultivation of Algae and Cyanobacteria
About...

Multi-Cultivator MC 1000-OD serves for small scale multiple cultivation of especially phototrophic organisms as algae, cyanobacteria and plant cells. Also other microorganisms as heterotrophic bacteria and yeast grown in suspension may enjoy this type of cultivator.

Key Features

  • Cultivation of autotrophic and heterotrophic microorganisms
  • Parallel or multi-variant experiments in 8 independent vessels
  • Optical density OD 680 and OD 720 reading
  • Standard white LED illumination

  • Optional multi-color illumination (up to 8 colors in each cultivation slot)
  • Turbidostatic cultivation
  • Online software control


Multi-Cultivator MC 1000-OD


Multi-Cultivator MC 1000-OD serves for small scale multiple-cultivation of especially phototrophic organisms as algae, cyanobacteria and plant cells. Also other microorganisms as heterotrophic bacteria and yeast grown in suspension may enjoy this type of cultivator.

Cultivation


Phototrophic suspended organisms and cells (algae, cyanobacteria, and plant tissue), heterotrophic suspended organisms (bacteria, yeast).
8 round glass test-tubes, each holding up to ca. 85 ml of cultivated suspension.
Silicon inert stopper with air sparging input and waste output.
Glass Aeration Tubes (standard) or Stainless-Steel Aeration Tubes (optional) that are easier and safer in manipulation.
Humidifier for a moistening of the sparging air to avoid an increased medium evaporation from the testing tubes.
Autoclavable design.
Three-Way Sampling Valve (optional) for culture sampling through the aeration tubing. Non-autoclavable.

Thermoregulation


Thermostated waterbath nesting 8 testing tubes.
Temperature range: from approximately 5oC above the ambient temperature up to 60oC (standard); 15 – 60oC with the Cooling Unit AC-700 (optional).
PWM pump for an automatic water refilling into the thermostated waterbath during the high-temperature or long-term cultivation (optional).

Illumination LED technology


Illumination up to 1.000 µmol. m-2. s-1 (optionally up to 2.500 µmol. m-2. s-1 with the Light Upgrade).
Single color version: cool white (standard); warm white, red, blue (optional).
Multi-color version (single color slots): 405, 450, 470, warm white, 540, 590, 660, 730 nm. Intensity: up to 1.000 µmol. m-2. s-1
Mix-color version (mixed color slots): 405, 450, 475, warm white, 530, 615, 660, 730 nm. Intensity: up to 2.500 µmol. m-2. s-1, i.e. around 300 µmol. m-2. s-1 per color per slot.

Illumination is independently adjustable for each test-tube in intensity, timing and modulation.
Constant light conditions or the alternation of various light and night cycles. The duration of the individual cycles: from seconds up to days. The mode of the light cycles: constant, linear increase or decrease, sinusoid mode for mimic of diurnal rhythms. Moreover, java scripting is incorporated in the Photobioreactor control software for a creation of the advanced light protocols (for example automatic light intensity adjustment in dependence on the actual biomass concentration).
Protective front cover that prevents cultivated samples to be influenced by an unwanted external light (sunshine).
NOTE: The lights are calibrated with a spherical sensor that is placed in the top third of the testing tube just in the middle. The light path of the testing tube is 27 mm.

Aeration system


Aeration system provides mixing and also air/CO2 supply to cultures.
The air sparging source: a small aeration pump providing total air flow rate around 1100 ml/min.
Manually adjustable valve manifold.
Glass Aeration Tubes (standard) or Stainless-Steel Aeration Tubes (optional) that are easier and safer in manipulation.
The aeration is interrupted during the OD measurement automatically to avoid any interference of the sparging bubbles with the OD measurement. That’s why the backpressure valve facilitates to release the residual air from the air sparging system before the OD measurement.
Incorporated module for compressed air/gas connection (valve + connectors; for tubing 6 mm in diameter).
Optionally, Multi-Cultivator can be linked to the Gas Mixing System GMS 150 that provides precise concentration and flow rate of the required gases.

OD monitoring


OD 680 and 720 is measured automatically for each testing tube.
The optical path of the measurement is 27 mm.
OD 720 measures light scattering on particles and can serve as a proxy for biomass growth whereas OD 680 is linked to the chlorophyll absorption and can be used for an estimation of chlorophyll concentration.
In the standard version, the optical density is periodically measured and the data are stored in the Multi-Cultivator internal memory. Stored data may later be downloaded to a PC and displayed in graphs or exported to TXT or Excel files via the provided OD View program.

The Photobioreactor control software (optional) enables remote online visualization of recorded data in real-time. Moreover, the software allows to fit the OD data with the various regression functions to facilitate the evaluation of the actual growth rate.

Turbidostatic cultivation


Turbidostatic Module (optional): control of the biomass concentration via OD 680 or OD 720 in each vessel independently. The OD can be regulated at a constant level or within a preset range depending on the experimental conditions.
The module includes its own pump for air sparging that is stronger compared to the standard air pump and thus can provide sufficient power to bubble the entire system properly.
Special silicon stoppers with an additional port for medium fed in.

Photobioreactor Control Software


Optionally, the entire Multi-Cultivator system and all its cultivation and monitoring functions (light, temperature, optical density, turbidostatic cultivation, and sparging gas composition) can be controlled via the Photobioreactor Control Software. The control software provides intuitive and dynamic interface for online monitoring and visualization of all measured data as well as creation of user-defined protocols. For more details see our website about the Photobioreactor Control Software.

Applications


  • High-throughput screening in multi-tubes experimental design
  • Bioprospecting of natural populations
  • Different organisms and mutants testing and comparing
  • Optimization of diverse cultivation conditions as nutrients, illumination, etc.
  • Regulatory profiling of algae
  • Growth dynamics monitoring
  • Optimizing yield of end-product
  • Toxicity tests

Technical Specification


Multi-Cultivator Versions


Version Standard Intensity /
Light Upgrade
Specification Description Light Colors
MC-1000-OD
STANDARD
1.000 / 2.500
µmol.m-2.s-1
single color slot identical light color
for all slots
cool white (standard);
warm white, blue, red (optional)
MC-1000-OD
MULTI
1.000 µmol.m-2.s-1 single color slot various light color
for each slot
405, 450, 470, warm white,
540, 590, 660, 730 nm
MC-1000-OD
MIX
2.500 µmol.m-2.s-1 mixed color slot combination of 8 light
color for each slot
405, 450, 475, warm white,
530, 615, 660, 730 nm


Multi-Cultivator MC-1000-OD

Standard single color version (usually cool white, optional colors for single-color device version: warm white, red 635 nm, blue 470 nm). Algae cultivator with 8 testing tubes and automatic optical density monitoring (OD 680 and 720 nm). Controlled parameters are light, temperature, and aeration.

Available colors: cool white (standard), warm white, blue or red (optional). Illumination intensity up to 1.000 µmol.m-2.s-1 (optionally up to 2.500 µmol.m-2.s-1 with the Light Upgrade).

Multi-Cultivator MC-1000-OD-MULTI


Multi-color device version. It is equipped with 8 test tubes; each tube being illuminated by light of different wavelength. Controlled parameters: light, temperature, aeration, and OD 680 and 720 nm.

Available colors (single color slots): UV-violet 405 nm, royal blue 450 nm, blue 470 nm, warm white, green 540 nm, yellow-orange 590 nm, deep red 660 nm, far red 730 nm. Illumination intensity up to 1.000 µmol.m-2.s-1.

NOTE: Wavelengths in nanometers show illustrative maximum peak values, but spectral coverage is wider for each color. The maximum peak values can differ a little bit.

Multi-Cultivator MC-1000-OD-MIX


This instrument version allows combining up to 8 different LED colors within each cultivation slot. Each color in each slot can be individually controlled and thus customized spectra may be defined by the user. Controlled parameters: light, temperature, aeration, and OD 680 and 720 nm.

Available colors (mix-color slots): UV-violet 405 nm, royal blue 450 nm, blue 475 nm, warm white, green 530 nm, red-orange 615 nm, deep red 660 nm, far red 730 nm. Illumination intensity up to 2.500 µmol.m-2.s-1 (i.e. a 300 µmol.m-2.s-1 for each color/slot).

NOTE: Wavelengths in nanometers show illustrative maximum peak values, but spectral coverage is wider for each color. The maximum peak values can differ a little bit.

Pricing


Multicultivator MC 1000 - OD
Version Characteristics Price
Multi-Cultivator
MC 1000-OD
Algae cultivator with 8 testing tubes and automatic optical density monitoring (OD 680 and 720 nm). Controlled parameters are light, temperature, and aeration. Included is also the module for air/gas connection. 5.500 €
Multi-Cultivator
MC 1000-OD-MULTI
Multi-color device version covering spectrum from 405 nm to 730 nm. Equipped with 8 test tubes; each tube being illuminated by light of different wavelength. Controlled parameters: light, temperature, aeration, and OD 680 and 720 nm. Includes module for air/gas connection. 6.500 €

Accessories

FMT 150/MC 1000 Control Unit
with Software Version BASIC *
Includes control unit (notebook with LCD, integrated keyboard and mouse) + BASIC version software for FMT 150/MC 1000 control,
user-defined protocol writing, data collection, and visualization in tables or graphs.
2.490 €
FMT 150/MC 1000 Control Unit
with Software Version ADVANCED *
Includes control unit (notebook with LCD, integrated keyboard, and mouse) + ADVANCED version software for FMT 150/MC 1000,
user-defined protocol writing, data collection, and visualization in tables or graphs.
2.990 €
Light Upgrade for Multi-Cultivator Enables reaching maximum intensity of illumination for each cultivation tube up to 2,500 µmol.m-2.s-1 Not available for the multi-color device versions MC-1000-OD-MULTI. 390 €
AC-700 Cooling Unit Enables reduction of the temperature in the water reservoir if compared to the ambient air temperature. At laboratory temperature around 22 °C, the temperature of the water bath can be lowered down to 15 °C with resolution of +/- 1 °C. This accessory is also valuable when high light intensities are required for cultivation as some heating of the water bath will occur from the high output of LED light. The unit is supplied with water pump AC 700, water chiller and connecting parts. 1.290 €
AC-700 Cooling Unit - Version 110 V/60 Hz AC-700 Cooling Unit that is intended for 110V and 60Hz AC electricity. It enables reduction of the temperature in the water reservoir if compared to the ambient air temperature. At laboratory temperature around 22 °C, the temperature of the water bath can be lowered down to 15 °C with resolution of +/- 1 °C. This accessory is also valuable when high light intensities are required for cultivation as some heating of the water bath will occur from the high output of LED light. The unit is supplied with water pump AC 700, water chiller and connecting parts. 1.290 €
Turbidostat Module TS-1100 Turbidostat Module is equipped with 8 valves and thus can serve all 8 testing tubes with independent control to each tube. Turbidostat is fully software controlled. 2.990 €
Gas Mixing System GMS 150 The standard version GMS 150 includes two channels (channel 1 for Air-N; channel 2 for CO2). Preferably it is intended for use with PSI Multi-Cultivators, small-volume Photobioreactors, large-volume Photobioreactors and Growth Chambers. 8.680 €
Gas Mixing System GMS 150-MICRO GGMS 150-MICRO includes two channels (channel 1 for Air-N; channel 2 for CO2); its maximum flow is 5l/min. Preferably it is intended for use with Multi-Cultivators and small-volume Photobioreactors. 5.990 €
PWM Water Pump Serves for automatic water refill to the water reservoir when the water level drops below the required level as detected by the water level sensor. Primarily, PWM Pump is recommended when high cultivation temperature is required in the water reservoir and faster water evaporation occurs. 680 €
Glass Test Tube for Cultivation * Glass test tube used for cultivation the Multi-Cultivator. 5 €
Set of 8 Standard Silicone Plugs * Set of 8 silicone plugs for cultivation tubes; standard plug
is equipped with two inlets.
49 €
Set of 8 Silicone Plugs with Additional Medium Inlet * Set of 8 silicone plugs for cultivation tubes. The plug is equipped with three inlets; the additional medium inlet comes with a short metal tube. The plug is suitable for the turbidostatic cultivation. 69 €
Set of 20 Glass Aeration Tubes * One set includes 20 long glass tubes used for aeration in the Multi-Cultivator test tubes. 25 €
Set of 10 Stainless-Steel Aeration Tubes * One set includes 10 stainless-steel tubes used for aeration in the Multi-Cultivator tubes. The tubes are made from stainless steel and they are autoclavable. Being easier and safer in manipulation, they may replace the standard glass tubes. 65 €
Set of 10 Three-Way Valves * The valve serves for sterile culture sampling during cultivation. 19 €
Spare Part Kit SPK1-MC * Kit includes: 9 glass test tubes, 20 long glass aeration tubes, 8 short Teflon tubes, and 8 silicone plugs. 95 €
Spare Part Kit SPK2-MC * Extended kit version includes: 9 glass test tubes, 20 long glass aeration tubes, 8 short Teflon tubes, 8 silicone plugs, silicon tubing 3/6 (6 m) and 5/8 (2 m), 1 air filter, 4 luer connector sets (8 tube fittings, 2 luer lock rings, 10 male luer lock rings, 10 female luer threads). 169 €
Protective Front Cover Black metal cover, which can easily be installed on the front part of the MC, gives protection against excessive LED radiation. 80 €
Spare Gas Dispenser Spare gas dispenser with 8 valves, luer connections and tubing. 490 €
User-Defined Custom Protocol When uploaded to the device, this Protocol can support up to 224 light phase intervals, each defined by light intensity and duration. Intended for MC device without external PC control. 400 €
Additional Protective Glasses * Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge. 19 €