Large-Scale PBRs
Precise Phototrophic Cultivation of Algae and Cyanobacteria
About...

PSI Large-Scale Photobioreactors are systems that create a controlled artificial environment for upscaling of photosynthetic microorganisms’ biomass production. Standard systems are based on flat cultivation vessel (25 l or 100 l in volume) whereas customized systems comprising range of various cultivation tanks that meets different customers’ requirements. The cultivation units is operated by the Control Unit placed inside the Storing Rack.

Key Features

  • 100 L or 25 L working volume; tanks may be cascaded.
  • In-built temperature regulation in the range from 30°C to 50°C (with ambient temperature around 20 °C)
  • Gas sparging (aeration)
  • Stainless steel and aluminium frame


  • Front and back and side windows made of thick glass
  • Gas tight vessel lid with gas and electronics fittings
  • Emptying valve
  • Inlets for substrate pumping and harvesting

Large-Scale Photobioreactors


PSI Large-Scale Photobioreactors are systems that create a controlled artificial environment for upscaling of photosynthetic microorganisms’ production and their metabolites. Standard systems are based on flat cultivation vessel (25 l or 100 l in volume) whereas customized systems comprising range of various cultivation tanks that meets customers’ requirements. The cultivation units is operated by the Control Unit placed inside the Storing Rack


Cultivation Vessel


  • Photoautotrophic suspended microorganisms, mostly algae and cyanobacteria.
  • Flat, rectangular glass cultivation vessel with a short light path (7 cm) for an optimal irradiance.
  • Different size: 25 l, 100 l
  • Stainless steel vessel lid with gas and electronics fittings.
  • Integrated Aeration System: autoclavable air sparger (with limited reuse).
  • Humidifier for a moistening of the sparging air to avoid an increased medium evaporation from the cultivation.
  • Emptying valve.




Thermoregulation


  • In-built temperature regulation in the range of 30 - 50 °C (with ambient temperature around 20 °C).
  • Cooling module (optional) widens the temperature range to 15 - 60 °C (ambient temperature not exceeding 30 °C).



Illumination


  • Bi-color light panel with separately controllable channels.
  • Cool white-red or cool white-blue. Other color combination on request.
  • Total illumination intensity up to 500 and 200 µmol. m-2. s-1 for white and red light, respectively.
  • Optionally, up to 2.000 µmol. m-2. s-1 with the Light Upgrade.
  • Light path: 70 mm.
  • Illumination is independently adjustable for light color in intensity, timing and modulation.
  • Constant light conditions or the alternation of various light and night cycles can be set. The duration of the individual cycles run from seconds up to days. The mode of the light cycles: constant, linear increase or decrease, sinusoid mode for mimic of diurnal rhythms. Moreover, java scripting is incorporated in the Photobioreactor Control Software for a creation of the advanced light protocols (for example an automatic light intensity adjustment depending on the actual biomass concentration).
  • Additional Protective Glasses: Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge.




Aeration System


  • Aeration system provides mixing and also air/CO2 supply to cultures.
  • The air sparging source (optional): Air pump supplemented with a valve, which interrupts bubbling before the OD and fluorescence measurements to avoid any bubble interference with the measurements and thus to allow for more precise readings.
  • Optionally, the Photobioreactor FMT 150 can be linked to the Gas Mixing System GMS 150 that provides precise concentration and flow rate of the required gases.



OD and Fluorescence monitoring


  • OD 680 and 720 is measured automatically.
  • OD 720 measures light scattering on particles and can serve as a proxy for biomass growth whereas OD 680 is linked to the chlorophyll absorption and can be used for an estimation of chlorophyll concentration.
  • Chlorophyll-a Fluorescence measurement with an integrated Double-Modulation Fluorometer. Blue and red excitation light. Determined parameters: F0, FT, FM, F’M, (F’M – FT/F’M).
  • The Photobioreactor Control Software (optional) enables remote online visualization of recorded data in real-time. Moreover, the software allows to fit the OD data with the various regression functions to facilitate the evaluation of the actual growth rate.
  • The optical path of OD and Chl-a fluorescence measurement: 10 mm.



Sensors


  • pH / Temperature Module includes the electronic module, Clark-type sensor and cable.
  • O2 Module (optional) to measure concentration of dissolved O2 (includes the electronic module, Clark-type sensor and cable).
  • CO2 Module (optional) to determine the dissolved carbon dioxide concentration in the medium (includes the electronic module, Clark-type sensor and cables).



Cultivation Strategies


  • Up to 8 peristaltic pumps (optional) can be linked to the Photobioreactor FMT 150 for supply of different nutrients, medium, catalysts, inhibitors, toxicants, base or acid, organisms, etc.
  • Batch cultivation
  • Fed-batch cultivation
  • Semi-continuous cultivation
  • Turbidostat Module (optional): The module enables continuous-flow turbidostatic cultivation. In the basic setup, the Photobioreactor optical sensing unit monitors the optical density (OD 680 or OD 720) of the culture. The Peristaltic Pump adds medium accordingly to keep the culture turbidity constant. The module includes one Peristaltic Pump PP 600 and supporting control software.
  • Chemostat Module (optional): In chemostat mode pH value of the cultivation media is continuously adjusted by acid or base addition to the cultivation vessel in order to keep pH level on defined level. The module includes two Peristaltic Pumps PP 600 and supporting control software. One pump serves adding acid, the second pump adds base.
  • pH-Stat cultivation through Gas Mixing System GMS 150 (optional): The Photobioreactor Control Software for [*]GMS 150 enables to create a Java script that will keep the pH of the cultivation medium on defined level through a regulated gaseous CO2 addition.
  • Anaerobic cultivation via gas-tight lid and N2 sparging.



Customized Large-Scales Photobioreactors


  • Vertical Tubular Airlift Photobioreactor is a modular system scalable between 100 – 1000 l. Ambient or artificial LED illumination. Monitoring of OD680, 720 and chlorophyll fluorescence. Additional probes: pH/T, dO2 , CO2
  • Stainless 1,000 l Photobioreactor Temperature controlled cultivation tank with the immersed LED lighting and integrated sparging system. Monitoring of OD680, 720 and chlorophyll fluorescence. Additional probes: pH/T, dO2 , CO2
  • Modular Helical Tubular Photobioreactor (250 l per one unit) with a degassing system. Ambient or artificial LED illumination. Monitoring of OD680, 720 and chlorophyll fluorescence. Additional probes: pH/T, dO2 , CO2




Photobioreactor Control Software


  • Optionally, the entire Photobioreactor FMT 150 system and all its cultivation and monitoring functions and accessories can be controlled via the Photobioreactor Control Software. The control software provides intuitive and dynamic interface for online monitoring and visualization of all measured data as well as creation of user-defined protocols. For more details see our website about the Photobioreactor Control Software.




Applications


  • Verification of cultivation upscaling
  • Biomass production
  • End-product yield optimization


Pricing


Large-Scale Photobioreactors
Version Characteristics Price
Photobioreactor System 100 L (Base) AFlat cultivation tank (100 L working volume) with integrated temperature regulation and bubble stirring of the culture 15.999 €
Photobioreactor System 25 L (Base) MFlat cultivation tank (25 L working volume) with integrated temperature regulation and bubble stirring of the culture 11.999 €

Accessories

Illumination Module for 100L PBR Bi-color, LED-based panel for 100 L Photobioreactor. Controllable in intensity, timing and modulation. Maximum intensity 700 µmol.m-2. s-1 (total for both colors). 3.999 €
Standard Illumination Module for 25L PBR Bi-color, LED-based panel for 25 L Photobioreactor. Controllable in intensity, timing and modulation. Maximum intensity 700 µmol.m-2. s-1 (total for both colors). 2.499 €
Enhanced Illumination Module for 25L PBR Enhanced illumination panel (bi-color, LED-based) for 25 L Photobioreactor. Controllable in intensity, timing and modulation. Maximum intensity 2,000 µmol.m-2. s-1 (total for both colors). 3.999 €
Electrode Module Enables connecting up to four measuring sensors to the Photobioreactor. Standard sensors: pH/T, dO2, CO2. Sensors are not included in the price of the Electrode Module; cable is included for each purchased sensor. 3.299 €
pH/T Sensor * Supplementary pH/T sensor for measuring pH in the cultivated suspension (without cable). 500 €
CO2 Sensor * CO2 Sensor for measuring dissolved carbon dioxide (without cable). 2.990 €
dO2 Sensor * Optical dO2 sensor for measuring dissolved oxygen (without cable). Included is also calibration kit. 2.490 €
Additional Cable to CO2 Sensor (25L and 100L)* Supplementary cable and connector to CO2 sensor. Includes also USB dongle for sensor calibration. 250 €
Additional Cable to O2 Sensor (25L and 100L)* Supplementary cable and connector to O2 sensor. Includes also USB dongle for sensor calibration. 590 €
Additional Cable to pH/T Sensor (25L and 100L) * Supplementary cable and connector to pH/T sensor. Includes also USB dongle for sensor calibration. 250 €
Optical Module Includes PAM fluorometer for measuring Ft and QY as well as turbidometer for measuring optical density at 680 nm and 735 nm. 4.999 €
Turbidostat Module Enables continuous cultivation of the culture (growth-associated parameters may be optical density or measured fluorescence). Includes one pump with needed accessories and software support. 3.799 €
Chemostat Module / pH Stat Module Includes two Peristaltic Pumps PP 600 and software support. One pump serves adding acid, the second pump adds base. The pumps may be controlled manually or by the software. 2.590 €
Cooling Module Enables widening the range of temperature for cultivation to 15-60 °C (ambient temperature must be around 20 °C). 3.799 €
Control Unit with a Storing Rack * Includes a PC with ADVANCED version software for control of the Photobioreactor and all its accessories as well as data collection and visualization. Included are also storing rack and separate touch screen positioned on the rack door. 5.499 €
Gas Mixing System GMS 150 The standard version GMS 150 includes two channels (channel 1 for Air-N; channel 2 for CO). Preferably it is intended for use with PSI Multi-Cultivators, small-volume Photobioreactors, large-volume Photobioreactors and Growth Chambers. 8.680 €
Additional Protective Glasses * Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge. 19 €